How to measure hematocrit manually

LAB POCKET GUIDE📒

Sometimes it happens that we have to measure the hematocrit (HCT) or packed cell volume (PCV) manually.💉

this can happen when:

  • we don’t have PCV igroscopic tubes,
  • we don’t have the reader (hawksley micro-haematocrit reader)
  • the sample is not suitable for the measurement

What will you need? 💪

  • 🧪whole blood sample (which can be either a hygroscopic tube or a normal one) CENTRIFUGATED,
  • 📏ruler
  • 🧮calcolator,

What to do then?

How to measure haematocrit manually

steps:

  1. measure total mm of plasma or serum
  2. measure mm of the red cell,
  3. therefor: mm(millimeters) red cells : mm total sample = x : 100
  4. calculate x = mm RED CELLS*100/ mm TOT

the result obtained is the calculated volume percentage of red blood cells (erythrocytes) in the blood = HEMATOCRIT.

HCT/PCV range:
dog = 37-55%
cat = 30-45%

The Lab Pocket Guide series collects everyday tips and small guides for veterinary technicians on laboratory, laboratory analysis, parasitology,